9CL8

Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.411, 89.698, 99.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.760 - 2.110
R-factor	0.16116
Rwork	0.160
R-free	0.19156
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.532
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.150
High resolution limit [Å]	2.110	5.720	2.110
Rmerge	0.073	0.029	0.501
Rmeas	0.080	0.032	0.542
Rpim	0.031	0.013	0.205
Total number of observations	270049
Number of reflections	41131	2243	2011
<I/σ(I)>	6.3
Completeness [%]	100.0	99.9	100
Redundancy	6.6	6.3	6.9
CC(1/2)	0.999	0.999	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	296	0.8 M NaCit, 0.1M HEPES pH 6.4, 60% glycerol, 10 mM DTT