9CJ0
The High-Resolution Structure of a Variable Lymphocyte Receptor from Petrozymon marinus Capable of Binding to the Brain Extracellular Matrix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-14 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.522, 53.137, 60.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.880 - 1.300 |
| R-factor | 0.18634 |
| Rwork | 0.185 |
| R-free | 0.22175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.636 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 36207 | 6984 |
| <I/σ(I)> | 20.1 | 3.3 |
| Completeness [%] | 98.0 | 94 |
| Redundancy | 8.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2.5-2.8 M ammonium sulfate, 2% hexyleneglycol, 100 mM HEPPS (pH 8) |
