9CIJ
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/L36R at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2021-11-20 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.031, 60.384, 38.303 |
| Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
| Resolution | 24.900 - 1.900 |
| R-factor | 0.16706 |
| Rwork | 0.165 |
| R-free | 0.20105 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.655 |
| Data reduction software | CrysalisPro (41.93a 64-bit (release 15-12-2020)) |
| Data scaling software | CrysalisPro (41.93a 64-bit (release 15-12-2020)) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.900 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.022 | 0.163 |
| Number of reflections | 11216 | 1127 |
| <I/σ(I)> | 40 | 5.04 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 35% MPD, 25mM Potassium Phosphate |
