9CII
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/L36R at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2021-08-04 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.172, 60.277, 38.350 |
| Unit cell angles | 90.00, 93.69, 90.00 |
Refinement procedure
| Resolution | 23.420 - 1.850 |
| R-factor | 0.16836 |
| Rwork | 0.166 |
| R-free | 0.20887 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.598 |
| Data reduction software | CrysalisPro (41.93a 64-bit (release 15-12-2020)) |
| Data scaling software | CrysalisPro (41.93a 64-bit (release 15-12-2020)) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.660 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.024 | 0.161 |
| Number of reflections | 12187 | 1225 |
| <I/σ(I)> | 36.95 | 4.91 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 30% MPD, 25mM Potassium Phosphate |
