9CHI
Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA-Y62A) heteromeric complex (bound to SAH - structure 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.050, 59.060, 108.580 |
| Unit cell angles | 89.95, 90.11, 85.97 |
Refinement procedure
| Resolution | 19.640 - 2.200 |
| R-factor | 0.26703 |
| Rwork | 0.265 |
| R-free | 0.30880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.448 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.640 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.090 | 0.390 |
| Number of reflections | 56505 | 7177 |
| <I/σ(I)> | 7.15 | |
| Completeness [%] | 86.3 | |
| Redundancy | 2.01 | |
| CC(1/2) | 0.995 | 0.931 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 240 mM sodium malonate pH 6, 12% PEG 3350 |
