9CFT

Adeno-associated virus serotype 6 basic regions in complex with importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	79.013, 89.319, 101.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.820 - 2.300
R-factor	0.2155
Rwork	0.214
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.488
Data reduction software	XDS (1.18.2_3874)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.211.5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.820	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	32611	316
<I/σ(I)>	10.5	2
Completeness [%]	99.8
Redundancy	5.6
CC(1/2)	0.997	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.65 M Na citrate, 0.01 M DTT, 0.1 M HEPES, pH 7.0