9CF7
Germline-targeting HIV-1 gp120 engineered outer domain eODgt8 in complex with Fab eOD-PL01.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 166.767, 166.767, 100.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.550 - 3.550 |
| Rwork | 0.265 |
| R-free | 0.29940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.994 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.550 | 3.890 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Rmerge | 0.260 | 3.100 |
| Rmeas | 0.274 | 3.250 |
| Rpim | 0.087 | 1.000 |
| Number of reflections | 10327 | 2421 |
| <I/σ(I)> | 9.1 | 1 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 9.5 | 9.9 |
| CC(1/2) | 0.997 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M potassium dihydrogen phosphate, 20% Peg3350 |
