9CF4
Crystal structure of the dimeric transaminase DoeD from C. salexigens DSM 3043.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.383, 94.102, 219.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.913 - 1.499 |
| R-factor | 0.113 |
| Rwork | 0.112 |
| R-free | 0.15120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.101 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.913 | 1.553 |
| High resolution limit [Å] | 1.499 | 1.499 |
| Rpim | 0.030 | 0.345 |
| Number of reflections | 136251 | 13274 |
| <I/σ(I)> | 17.2 | 1.93 |
| Completeness [%] | 99.7 | 98.12 |
| Redundancy | 10.2 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 0.1 M TRIS pH 8, 19.28 % (w/v) PEG 6000 and 0.3 M MgCl2 |
