9CE6

Key structural role for the conserved cis-proline of soybean serine hydroxymethyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	293
Detector technology	CMOS
Collection date	2024-04-04
Detector	RDI CMOS_8M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	116.603, 129.685, 58.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.340 - 2.250
R-factor	0.1946
Rwork	0.191
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.938
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.570	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.080	2.437
Rpim	0.044	1.354
Number of reflections	43034	3891
<I/σ(I)>	13.5	0.9
Completeness [%]	100.0	100
Redundancy	7.9	8.1
CC(1/2)	0.999	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Hepes, pH 7.5 20% w/v PEG 3350 2% v/v tacsimate, pH 7.0