9CD3
Cryo-EM structure of Candidatus Saccharibacterium phosphoketolase complexed with thiamine diphosphate
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | TFS Selectris X |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.18 |
| Number of Particle | 4115482 |
| Software | cryoSPARC,PHENIX,EPU,Coot,UCSF ChimeraX,ISOLDE |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
