9CBF
Crystal Structure of Danio rerio Histone Deacetylase 10 in Complex with m-Aminomethyl Phenylthioketone
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-03-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 80.411, 80.411, 236.953 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.350 - 2.260 |
R-factor | 0.2363 |
Rwork | 0.235 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.481 |
Data reduction software | autoPROC (1.0.5) |
Data scaling software | Aimless (0.7.13) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.21rc1_5156) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.350 | 2.330 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.123 | 2.330 |
Rpim | 0.043 | 0.770 |
Number of reflections | 39402 | 3873 |
<I/σ(I)> | 9.1 | 1 |
Completeness [%] | 91.7 | 100 |
Redundancy | 9.3 | 10 |
CC(1/2) | 0.998 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10 mg/mL HDAC10 protein, 2 mM inhibitor, 0.2 M KH2PO4, 20% w/v PEG 3350 |