9CB6
Crystal Structure of RT-PhyR (ruthe_02744)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-05 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920105 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.101, 149.543, 73.564 |
| Unit cell angles | 90.00, 101.24, 90.00 |
Refinement procedure
| Resolution | 33.850 - 2.830 |
| R-factor | 0.2348 |
| Rwork | 0.233 |
| R-free | 0.27190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.637 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.850 | 2.870 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.225 | 1.417 |
| Rmeas | 0.263 | 1.689 |
| Rpim | 0.135 | 0.910 |
| Number of reflections | 30415 | 1502 |
| <I/σ(I)> | 2.7 | 0.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.4 |
| CC(1/2) | 0.980 | 0.361 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | 0.1M Bis-tris propane, 0.2M KSCN, 20% PEG3350 |
