9CA5
Rns pocket mutant - H20A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979351 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.730, 97.930, 136.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.100 - 2.900 |
| R-factor | 0.2694 |
| Rwork | 0.264 |
| R-free | 0.31490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.749 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 13705 | 1448 |
| <I/σ(I)> | 3.08 | |
| Completeness [%] | 92.3 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.986 | 0.074 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1:1 v/v DMSO, 0.1 M succinnic acid, 14% PEG 3350, 0.03 M glycyl-glycyl-gylcine |
