9CA2

ENGINEERING THE HYDROPHOBIC POCKET OF CARBONIC ANHYDRASE II

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	42.700, 41.700, 73.000
Unit cell angles	90.00, 104.60, 90.00

Refinement procedure

Resolution	6.500 * - 2.800
R-factor	0.183 *
RMSD bond length	0.004 *
RMSD bond angle	2.100 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	6.500 *
High resolution limit [Å]	2.800 *
Total number of observations	13723 *
Number of reflections	7413 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	0.3 (mM)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		150 (mM)
4	1	drop		3 (mM)
5	1	reservoir	Tris-HCl	50 (mM)
6	1	reservoir		150 (mM)
7	1	reservoir		3 (mM)
8	1	reservoir	ammonium sulfate	2.0 (M)