9C9R

Crystal structure of Chaetomium thermophilum Gcn2 HisRS-like domain, F1090C/G1326S variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	82.111, 52.714, 136.891
Unit cell angles	90.00, 104.27, 90.00

Refinement procedure

Resolution	45.710 - 2.320
R-factor	0.2006
Rwork	0.199
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.868
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.710	2.320
High resolution limit [Å]	2.316	2.316
Number of reflections	95403	49637
<I/σ(I)>	6.41	0.96
Completeness [%]	99.3
Redundancy	8.5
CC(1/2)	0.992	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM HEPES pH 7.5, 15% v/v ethylene glycol and 10% v/v PEG 8000