9C9Q

Crystal structure of Chaetomium thermophilum Gcn2 HisRS-like domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	81.431, 52.003, 136.427
Unit cell angles	90.00, 104.14, 90.00

Refinement procedure

Resolution	35.530 - 2.000
R-factor	0.1976
Rwork	0.196
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.530	2.060
High resolution limit [Å]	2.000	2.000
Number of reflections	68536	3881
<I/σ(I)>	19.2	0.72
Completeness [%]	98.1
Redundancy	3.3
CC(1/2)	0.993	0.286

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M ammonium sulfate, 100 mM sodium cacodylate pH 6.5, 30% w/v polyethylene glycol (PEG) 8000.