9C7O

Crystal Structure of the Maize R ACT-like Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97849
Spacegroup name	P 43 21 2
Unit cell lengths	74.124, 74.124, 90.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.341 - 2.292
R-factor	0.2136
Rwork	0.209
R-free	0.26020
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.205
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.330
High resolution limit [Å]	2.290	6.210	2.290
Rmerge	0.133	0.095	0.425
Rmeas	0.158	0.098	0.444
Rpim		0.022	0.123
Number of reflections	11190	668	398
<I/σ(I)>	3.9
Completeness [%]	94.4	97.1	68.9
Redundancy	18.7	19.9	9.1
CC(1/2)		0.995	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M Sodium Thiocyanate, 20%PEG 3350