9C7F

Human monoclonal antibody MAD24-01 bound to the N-terminus of cleaved circumsporozoite protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	74.541, 88.320, 168.952
Unit cell angles	90.00, 89.75, 90.00

Refinement procedure

Resolution	45.000 - 1.820
R-factor	0.2119
Rwork	0.211
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.821
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.840
High resolution limit [Å]	1.820	1.820
Rmerge	0.183	1.024
Rpim	0.079	0.482
Number of reflections	192978	9564
<I/σ(I)>	3.6
Completeness [%]	97.6
Redundancy	3.2
CC(1/2)	0.995	0.306

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	20% PEG 3350 and 0.2 M potassium fluoride