9C7D

Human monoclonal antibody MAD22-38 bound to the N-terminus of cleaved circumsporozoite protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-18
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	73.803, 76.355, 88.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.400 - 1.990
R-factor	0.1861
Rwork	0.184
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.357
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.400	2.030
High resolution limit [Å]	1.990	1.990
Rmerge	0.160	1.625
Rpim	0.067	0.677
Number of reflections	34343	1703
<I/σ(I)>	6.44	2.4
Completeness [%]	97.9
Redundancy	6.6
CC(1/2)	0.989	0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	20% PEG 3350, 0.2 M ammonium chloride