9C7B

Crystal structure of R150H neurodevelopmental disease-associated U2AF2 variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	43.722, 55.793, 76.675
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.980 - 1.400
R-factor	0.1366
Rwork	0.134
R-free	0.16710
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6xlw
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.20.1)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.340	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.077	0.434
Rpim	0.032	0.219
Number of reflections	69899	1611
<I/σ(I)>	11.1	2.1
Completeness [%]	98.3	82.6
Redundancy	6.5	4.8
CC(1/2)	0.998	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	24 M sodium malonate pH 7.0, 5% w/v sucrose, 25% w/v PEG 3350, with addition of 0.1 uL of 5% w/v LDAO detergent