9C79
Human monoclonal antibody MAD21-101 bound to the N-terminus of cleaved circumsporozoite protein
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-16 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.493, 73.892, 86.573 |
| Unit cell angles | 90.00, 104.13, 90.00 |
Refinement procedure
| Resolution | 41.980 - 1.460 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.480 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.097 | 1.223 |
| Rpim | 0.041 | 0.625 |
| Number of reflections | 81657 | 4113 |
| <I/σ(I)> | 6 | 2.2 |
| Completeness [%] | 99.4 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.995 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 2.0 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M cacodylate |
