9C74
superfolder Green Fluorescent Protein with meta-nitro-tyrosine incorporated at position 66
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-10-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.809, 37.967, 68.464 |
| Unit cell angles | 90.00, 112.73, 90.00 |
Refinement procedure
| Resolution | 63.150 - 1.510 |
| R-factor | 0.1729 |
| Rwork | 0.170 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.165 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.150 | 1.530 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Number of reflections | 55986 | 1508 |
| <I/σ(I)> | 16.4 | 2.5 |
| Completeness [%] | 97.7 | 90.1 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.999 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1% tryptone, 0.001 M NaN3, 0.05 M Hepes pH 7.0, 20% w/v PEG350 |
