9C6T
Structure of the Human ISM1 TSR-AMOP domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.255, 114.255, 168.221 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.470 - 3.410 |
| R-factor | 0.3334 |
| Rwork | 0.332 |
| R-free | 0.36590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.734 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.470 | 49.470 | 3.680 |
| High resolution limit [Å] | 3.410 | 9.020 | 3.410 |
| Rmerge | 0.067 | 0.025 | 3.417 |
| Rmeas | 0.073 | 0.028 | 3.736 |
| Rpim | 0.030 | 0.012 | 1.464 |
| Total number of observations | 89619 | 4562 | 18746 |
| Number of reflections | 17232 | 953 | 3554 |
| <I/σ(I)> | 9.3 | 47.3 | 0.3 |
| Completeness [%] | 96.4 | 89.7 | 97.8 |
| Redundancy | 5.2 | 4.8 | 5.3 |
| CC(1/2) | 0.999 | 0.999 | 0.381 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289.15 | 1.0 M NaMelonate, pH 7.0 |
