9C6L
Yasminevirus c12orf29, a 5' to 3' RNA ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.897, 84.221, 115.240 |
| Unit cell angles | 90.00, 106.88, 90.00 |
Refinement procedure
| Resolution | 46.410 - 2.700 |
| R-factor | 0.2341 |
| Rwork | 0.232 |
| R-free | 0.27490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.543 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.190 | 0.110 | 1.229 |
| Rmeas | 0.200 | 0.116 | 1.328 |
| Rpim | 0.061 | 0.035 | 0.488 |
| Total number of observations | 550364 | ||
| Number of reflections | 55600 | 2925 | 2382 |
| <I/σ(I)> | 4.7 | ||
| Completeness [%] | 98.0 | 99 | 85 |
| Redundancy | 9.9 | 10.9 | 6 |
| CC(1/2) | 0.963 | 0.974 | 0.527 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.85 M sodium malonate, 50 mM NaCl, 0.1 M Bis-Tris, 20% ethylene glycol |
