9C68

The CRISPR associated CARF-adenosine deaminase Cad1-CARF in the cA6 bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9793
Spacegroup name	P 62 2 2
Unit cell lengths	85.925, 85.925, 188.721
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.960 - 1.820
R-factor	0.2703
Rwork	0.268
R-free	0.31230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.426
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.410	1.860
High resolution limit [Å]	1.820	1.820
Rmerge	0.038	2.017
Rmeas	0.053	2.853
Rpim	0.038	2.017
Total number of observations	69781	4137
Number of reflections	37752	2181
<I/σ(I)>	9.5	0.4
Completeness [%]	100.0
Redundancy	1.8	1.9
CC(1/2)	0.999	0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
Cad1CARF-cA4	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.1 M sodium acetate pH 4.5, 35 % (v/v) MPD