9C66
Structure of the Mena EVH1 domain bound to the polyproline segment of PTP1B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97932 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.654, 49.072, 54.105 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 1.400 |
R-factor | 0.1877 |
Rwork | 0.187 |
R-free | 0.19610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.472 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.21rc1_5156)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.830 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.095 | 0.577 |
Rmeas | 0.100 | 0.606 |
Rpim | 0.029 | 0.180 |
Total number of observations | 259048 | 12450 |
Number of reflections | 21372 | 1040 |
<I/σ(I)> | 18.8 | 6.3 |
Completeness [%] | 99.6 | |
Redundancy | 12.1 | 12 |
CC(1/2) | 0.998 | 0.889 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 2.0 M Ammonium sulfate and 0.1 M Bis-Tris |