9C5F

Crystal Structure Analysis of human PRKCB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	144.123, 144.123, 70.369
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.300 - 2.410
R-factor	0.2142
Rwork	0.213
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.645
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.300	2.480
High resolution limit [Å]	2.410	2.410
Rmerge	0.102
Rmeas	0.107
Rpim	0.033
Number of reflections	32787	2664
<I/σ(I)>	11.09	0.71
Completeness [%]	100.0
Redundancy	10.2	10.6
CC(1/2)	0.999	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6 M Ammonium sulfate, 100 mM Tris, pH 8.0