9C4U
Menin mutant T349M in complex with MLL peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-04-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.045, 80.235, 125.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.153 - 1.569 |
| Rwork | 0.160 |
| R-free | 0.19710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.852 |
| Data reduction software | HKL-2000 (722) |
| Data scaling software | HKL-2000 (722) |
| Phasing software | MOLREP (11.0 / 22.07.2010) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.569 | 1.569 |
| Rmerge | 0.150 | 0.963 |
| Number of reflections | 69845 | 3438 |
| <I/σ(I)> | 20.952 | 2.03 |
| Completeness [%] | 100.0 | |
| Redundancy | 7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.2 M lithium sulfate, 0.1 M HEPES, pH 7.5, 25% (w/v) PEG-3,350 |
