9C4T

menin mutant M327I in complex with MLL peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2023-04-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	49.075, 80.456, 124.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.131 - 1.461
Rwork	0.156
R-free	0.18440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.995
Data reduction software	HKL-2000 (722)
Data scaling software	HKL-2000 (722)
Phasing software	MOLREP (11.0 / 22.07.2010)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.490
High resolution limit [Å]	1.460	1.460
Rmerge	0.082	0.726
Number of reflections	86122	4168
<I/σ(I)>	28.13	2.27
Completeness [%]	99.5	97.1
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.2 M lithium sulfate, 0.1 M HEPES, pH 7.5, 25% (w/v) PEG-3,350