9C46

Right-left hybrid parallel G-quadruplex from SLC2A1 promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	196
Detector technology	PIXEL
Collection date	2023-07-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.920105
Spacegroup name	C 1 2 1
Unit cell lengths	64.137, 31.491, 31.500
Unit cell angles	90.00, 104.31, 90.00

Refinement procedure

Resolution	25.100 - 2.390
R-factor	0.2345
Rwork	0.233
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.668
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.523	2.429
High resolution limit [Å]	2.388	2.388
Rmerge	0.103	1.383
Number of reflections	2469	120
<I/σ(I)>	8.4
Completeness [%]	98.7	100
Redundancy	3.3	3.4
CC(1/2)	1.000	0.541

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	285	0.08 M NaCl 0.04 M Na Cacodylate pH 6.0 45% MPD