9C2K
The crystal structure of HIV-1 Rev Response Element Stem-Loop II in complex with a Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.359, 76.238, 82.599 |
| Unit cell angles | 116.56, 94.91, 102.75 |
Refinement procedure
| Resolution | 45.330 - 2.420 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.760 | 2.450 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.084 | 0.392 |
| Rmeas | 0.118 | 0.554 |
| Rpim | 0.083 | |
| Number of reflections | 102736 | 2744 |
| <I/σ(I)> | 9.4 | 1 |
| Completeness [%] | 98.0 | 94.4 |
| Redundancy | 1.8 | 1.8 |
| CC(1/2) | 0.978 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M Sodium acetate trihydrate pH 7.0, 20% PEG 3350 |
