9C23
Cyan thermostable protein (CTP) 1.0 at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-05 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.345, 77.305, 81.291 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.330 - 1.700 |
| R-factor | 0.1843 |
| Rwork | 0.182 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.910 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.330 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.112 | 0.763 |
| Rmeas | 0.125 | |
| Rpim | 0.055 | 0.498 |
| Number of reflections | 28036 | 8909 |
| <I/σ(I)> | 14.3 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 6.1 |
| CC(1/2) | 0.999 | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294.15 | 0.05 M Magnesium Chloride Hexahydrate 0.1 M Tris HCl pH 6.5 27.5% PEG 4000 |
