9C11
Crystal structure of Staphylococcal nuclease variant Delta+PHS L36R at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU PhotonJet-R |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2023-11-14 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.183, 53.201, 107.422 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.760 - 1.950 |
R-factor | 0.21313 |
Rwork | 0.211 |
R-free | 0.26094 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.764 |
Data reduction software | CrysalisPro (1.171.42.95a) |
Data scaling software | CrysalisPro (1.171.42.95a) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.210 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.015 | 0.193 |
Number of reflections | 22934 | 2297 |
<I/σ(I)> | 104.39 | 5.88 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 9.6 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 28% MPD 25mM K Phosphate |