9C0Y

Clathrin terminal domain complexed with Pitstop 2c

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-21
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	71.562, 73.674, 85.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.590 - 1.400
R-factor	0.18525
Rwork	0.184
R-free	0.20584
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.958
Data reduction software	DIALS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.590	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.070	1.217
Number of reflections	88731	8546
<I/σ(I)>	15.86
Completeness [%]	99.5
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG 3350, 150 MM POTASSIUM ACETATE, 0.1 M TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature