9C0X

Crystal structure of chimeric hemagglutinin cH11/1 in complex with broad protective antibody 31.b.09

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 21 3
Unit cell lengths	169.045, 169.045, 169.045
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 4.350
R-factor	0.3373
Rwork	0.335
R-free	0.38200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.034
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	4.420
High resolution limit [Å]	4.350	4.350
Number of reflections	10863	10839
<I/σ(I)>	6.1
Completeness [%]	100.0
Redundancy	13.5
CC(1/2)	0.990	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium phosphate dibasic, 20% w/v PEG3350