9C0O
Crystal structure of DmCfp1 PHD finger bound to H3K4me3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-03-07 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.270, 43.120, 47.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.950 - 1.530 |
R-factor | 0.158 |
Rwork | 0.156 |
R-free | 0.19360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.713 |
Data reduction software | xia2 |
Data scaling software | Aimless (0.7.15) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.950 | 1.585 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.134 | 1.372 |
Rmeas | 0.140 | 1.425 |
Rpim | 0.039 | 0.381 |
Number of reflections | 10748 | 1046 |
<I/σ(I)> | 11.6 | 2.1 |
Completeness [%] | 99.5 | 98.87 |
Redundancy | 12.8 | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 15% PEG3350, 0.01M Tris pH8.5, 0.2M Ammonium sulfate |