9BYE

Nanoparticle Crystal Structure of a Thermostabilized Mutant E.coli Ferritin ECFTNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	F 4 3 2
Unit cell lengths	180.373, 180.373, 180.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.380 - 1.750
R-factor	0.1794
Rwork	0.178
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.380	1.780
High resolution limit [Å]	1.750	1.750
Rmeas	0.095
Number of reflections	25810	1268
<I/σ(I)>	7.9
Completeness [%]	100.0
Redundancy	3.8
CC(1/2)	1.000	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M HEPES pH 7, 1% w/v PEG MME 2K, 1M Na2 Succ