9BXP

Nanoparticle Crystal Structure of a Thermostabilized Mutant of an As-Isolated FtnA (Ferritin) from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	I 4
Unit cell lengths	123.413, 123.413, 173.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.590 - 2.330
R-factor	0.1972
Rwork	0.196
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.788
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.590	2.413
High resolution limit [Å]	2.330	2.330
Number of reflections	51690	4541
<I/σ(I)>	9.6
Completeness [%]	97.2
Redundancy	4.8
CC(1/2)	0.851	0.322

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.4	293	2.8 M Ammonium Sulfate, 0.1 M Sodium Citrate pH 4.4