9BXH

OvsM from Marinimicrobium koreense, a SAM-dependent N-methyltransferase involved in ovoselenol biosynthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-19
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9686
Spacegroup name	C 2 2 21
Unit cell lengths	83.838, 90.058, 80.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.000 - 1.480
R-factor	0.1772
Rwork	0.176
R-free	0.19640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.776
Data reduction software	XDS (XDS-Apple_M1)
Data scaling software	XDS (XDS-Apple_M1)
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	1.533
High resolution limit [Å]	1.480	1.480
Rmerge	0.029	0.679
Rmeas	0.041	0.960
Rpim	0.029	0.679
Number of reflections	50394	4614
<I/σ(I)>	11.38	1.1
Completeness [%]	98.9	90.99
Redundancy	2	2
CC(1/2)	0.999	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M HEPES, pH 7.0, 5% v/v Tacsimate, pH 7.0, 10% w/v PEG5000 MME