9BWA

Crystal structure of the Transport and Golgi Organization protein 2 Homolog (TANGO2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-13
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	50.011, 50.171, 241.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.970 - 1.700
R-factor	0.1647
Rwork	0.164
R-free	0.18660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_5295: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.108	2.292
Rmeas	0.112	2.380
Rpim	0.031	0.638
Total number of observations	900003	49944
Number of reflections	68343	3633
<I/σ(I)>	13.7	1.5
Completeness [%]	100.0
Redundancy	13.2	13.7
CC(1/2)	0.999	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	Grid Salt HT screen C9: 1.0 M sodium phosphate monobasic monohydrate / potassium phosphate dibasic pH 6.9, TANGO2 at 11.4 mg/mL. plate 14002 well E10 drop 2. Puck: PSL-1404, Cryo: 80% (v/v) crystallant and 20% (v/v) glycerol