9BW0
RNA Polymerase II - No ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 219.090, 390.920, 281.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.080 - 3.510 |
| R-factor | 0.2197 |
| Rwork | 0.219 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.712 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.080 | 3.550 |
| High resolution limit [Å] | 3.510 | 3.510 |
| Number of reflections | 133630 | 1119 |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 90.0 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.920 | 0.280 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Tacsimate, Hepes pH 7.0 |
