9BUJ

Structure of PfPL1 from Pseudoalteromonas fuliginea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-10
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	48.504, 58.137, 149.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 2.190
R-factor	0.1853
Rwork	0.182
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.075
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.190	2.190
Rmeas	0.103	0.320
Rpim	0.048	0.168
Number of reflections	21844	900
<I/σ(I)>	14.4
Completeness [%]	97.8
Redundancy	4.2
CC(1/2)	0.984	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	20% PEG 3350, 0.1M sodium malonate pH7.0