9BUF
Hydrolase domain of Chitinase 1 from Streptomyces venezuelae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 77.529, 54.769, 144.421 |
| Unit cell angles | 90.00, 92.13, 90.00 |
Refinement procedure
| Resolution | 41.570 - 1.750 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.884 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.570 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.244 | 2.302 |
| Rmeas | 0.264 | 2.485 |
| Rpim | 0.101 | 0.929 |
| Total number of observations | 416395 | 23362 |
| Number of reflections | 61279 | 3341 |
| <I/σ(I)> | 6.2 | 1 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.993 | 0.504 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30 % (w/v) PEG 2000 MME, 0.2 M Ammonium sulphate, 0.1 M Sodium acetate |
