9BTM

NRas 1-169 Q61R in Complex with Shoc2 80-582

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	78.884, 89.339, 119.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.880 - 2.730
R-factor	0.2037
Rwork	0.202
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.497
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.580	2.741
High resolution limit [Å]	2.700	2.732
Rmerge	0.160
Number of reflections	23050	225
<I/σ(I)>	13.2	0.7
Completeness [%]	100.0	100
Redundancy	15	6.9
CC(1/2)	0.998	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.1 M Hepes pH 7, 15% PEG 4000