9BR7
Crystal structure of human succinyl-CoA:glutarate-CoA transferase (SUGCT) in complex with Losartan carboxylic acid
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-11-08 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.920105 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.933, 119.809, 136.246 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.060 - 2.080 |
R-factor | 0.2214 |
Rwork | 0.220 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.476 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.310 | 2.120 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.134 | 1.882 |
Rmeas | 0.150 | 2.101 |
Rpim | 0.067 | 0.923 |
Total number of observations | 513724 | 26323 |
Number of reflections | 106315 | 5254 |
<I/σ(I)> | 6.8 | 0.9 |
Completeness [%] | 99.8 | |
Redundancy | 4.8 | 5 |
CC(1/2) | 0.997 | 0.420 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350 |