9BQ4
YTP-E E148D, a weakly yellow thermostable protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-23 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.784, 137.777, 74.556 |
Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
Resolution | 20.320 - 3.000 |
R-factor | 0.2267 |
Rwork | 0.221 |
R-free | 0.28070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.672 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (8.0.006) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.320 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.104 | |
Rpim | 0.066 | 0.133 |
Number of reflections | 16717 | 2738 |
<I/σ(I)> | 11.5 | 5.7 |
Completeness [%] | 97.5 | 99.8 |
Redundancy | 4.8 | 5.6 |
CC(1/2) | 0.986 | 0.970 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 0.2 M Magnesium chloride hexahydrate, 0.1 M Tris hydrochloride pH 8.5, 35% PEG 4000 |