9BP2

The death domain (DD) of the human mucosa associated lymphoid tissue lymphoma translocation protein 1 (MALT1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	I 2 2 2
Unit cell lengths	34.527, 79.315, 86.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.210 - 2.010
R-factor	0.2219
Rwork	0.218
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.612
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.21_5207)
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.210	2.090
High resolution limit [Å]	2.010	2.010
Rmerge	0.014
Rmeas	0.019
Rpim	0.014	0.157
Number of reflections	12166	7726
<I/σ(I)>	34.44
Completeness [%]	78.9
Redundancy	1.6
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	293	PEG3350 20% MgCl2 0.2 M MES 0.1 M (pH6)