9BON
Crystal structure of glucosyltransferase (GTD) domain of TpeL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.093, 64.730, 121.181 |
| Unit cell angles | 90.00, 102.03, 90.00 |
Refinement procedure
| Resolution | 73.445 - 2.222 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.008 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549: ???) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.520 | 10.310 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.080 | |
| Rmeas | 0.090 | |
| Rpim | 0.042 | |
| Number of reflections | 56042 | 2780 |
| <I/σ(I)> | 11.2 | 2.1 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 4.6 | |
| CC(1/2) | 0.997 | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M potassium sodium tartrate and 20% (w/v) PEG 3350 |
