9BOK
Crystal structure of reduced bovine trypsin, 50mM DTT-treated
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 54.428, 54.428, 107.156 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.380 - 1.350 |
R-factor | 0.1715 |
Rwork | 0.170 |
R-free | 0.19230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.807 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.064 | 0.786 |
Number of reflections | 40294 | 1866 |
<I/σ(I)> | 23.94 | |
Completeness [%] | 97.1 | |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M Imidazole pH 7.0, 30% PEG-8000, 0.3M ammonium sulfate |