9BNS
Rhesus macaque ITS114.01 Fab in complex with SIV MPER peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 175.900, 175.900, 205.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.440 - 3.000 |
| R-factor | 0.2304 |
| Rwork | 0.228 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.562 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_5278) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.440 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 36532 | 5184 |
| <I/σ(I)> | 7.33 | |
| Completeness [%] | 96.2 | |
| Redundancy | 4.8 | |
| CC(1/2) | 0.995 | 0.194 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | AmSO4 |
